Acid Red 94
Rose Bengal sodium salt
CAS: 632-69-9
Molecular Formula: C20H5Cl4I4NaO5
Acid Red 94 - Names and Identifiers
| Name | Rose Bengal sodium salt |
| Synonyms | r105sodium Acid Red 94 oxanthone2na rosebengalsodium Rose bengal Na salt Rose Bengal sodium salt foodredno.105,sodiumsalt foodredcolorno.105,sodiumsalt 4',5',7'-tetraiodo-droxy-2disodiumsalt 2,4,5,7-Tetraido(m,p,o,m)tetrachlorofluorescein,disodium salt 4,5,6,7-Tetrachloro-2,4,5,7-tetraiodofluorescein disodium salt orescein, 4,5,6,7-tetrachloro-2,4,5,7-tetraiodo-, disodium salt 4,5,6,7-tetrachloro-2',4',5',7'-tetraiodo-fluoresceidisodiumsalt 9-(3',4',5',6'-tetrachloro-o-carboxyphenyl)-6-hydroxy-2,4,5,7-tetraiodo-3-is Disodium 3,4,5,6-tetrachloro-2-(2,4,5,7-tetraiodo-6-oxido-3-oxoxanthen-9-yl)benzoate disodium 4,5,6,7-tetrachloro-2,4,5,7-tetraiodo-3-oxo-3H-spiro[2-benzofuran-1,9-xanthene]-3,6-diolate |
| CAS | 632-69-9 |
| EINECS | 211-183-3 |
| InChI | InChI=1/C20H4Cl4I4O5.2K.Na/c21-10-8(9(20(31)32)11(22)13(24)12(10)23)7-3-1-5(25)16(29)14(27)18(3)33-19-4(7)2-6(26)17(30)15(19)28;;;/h1-2,29H,(H,31,32);;;/q;3*+1/p-2 |
Acid Red 94 - Physico-chemical Properties
| Molecular Formula | C20H5Cl4I4NaO5 |
| Molar Mass | 997.66 |
| Melting Point | >300°C |
| Water Solubility | soluble |
| Solubility | H2O: soluble1mg/mL |
| Appearance | Form Solid, color Red-brown |
| Color | Red-brown |
| Maximum wavelength(λmax) | 548 nm |
| Merck | 14,8262 |
| BRN | 3645857 |
| pKa | 3.9, 4.7(at 25℃) |
| Storage Condition | Store at +15°C to +30°C. |
| Sensitive | Light Sensitive |
| MDL | MFCD00005043 |
| Physical and Chemical Properties | Melting point> 300°C color index 45440 water soluble Sensitive Light Merck 14,8262 BRN 3645857 |
| Use | Used as an adsorption indicator |
Acid Red 94 - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Risk Codes | R36 - Irritating to the eyes R36/38 - Irritating to eyes and skin. |
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. S37/39 - Wear suitable gloves and eye/face protection S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
| UN IDs | UN 3264 8 / PGIII |
| WGK Germany | 3 |
| RTECS | LM5920000 |
| FLUKA BRAND F CODES | 8 |
| TSCA | Yes |
| HS Code | 32129000 |
| Toxicity | LD50 6.48g/kg(雄小鼠，经口)。 |
Acid Red 94 - Upstream Downstream Industry
| Raw Materials | Sodium chloride Acid Red 92 Tetrachlorofluorescein 3,4,5,6-TETRACHLOROFLUORESCEIN Tetrachlorophthalic anhydride Resorcinol |
Acid Red 94 - Reference
| Reference Show more | 1. Zeng Yongfang, Li Shiyan, Li Yan. Simultaneous determination of 12 synthetic pigments in wine by solid phase extraction-ultra performance liquid chromatography [J]. Chinese Journal of Health inspection, 2015,25(16):2689-2692. 2. [IF = 5.076] Xia Xia et al.. "Nanomaterials-Basel. 2018 Apr;8(4):234 3. [IF = 4.32] Shijuan Wu et al." Reduction of tellurite in Pinella sp. WSJ-2. Int Biodeter Biodegr. Oct;144:104751 |
Acid Red 94 - Introduction
Soluble in water, the aqueous solution is blue-red, and the sulfuric acid medium is brown. &&λmax:549 nm
Last Update：2022-10-16 17:27:31
Acid Red 94 - Reference Information
| content analysis | accurately weigh about 1g of sample, dissolve with water and fix the volume to 100ml, take 50ml of the liquid as sample liquid, and measure according to method 3 in the OT-15. |
| toxicity | LD50 6.48 g/kg (male mice, oral). |
| chemical properties | recombinant human TNFα has certain tolerance to pH and heat. At pH 6-10 and 4 ℃, it was still active after 24 hours. At 60 ℃, it still has activity after 1h. The TNFβ produced by Escherichia coli is a non-glycosylated protein, which is easy to condense under denaturation conditions, is not sensitive to trypsin, chymotrypsin and other enzymes, is unstable to heat, and is inactivated at 75 ℃, but its biochemical characteristics are similar to that of natural TNFβ. |
| use | is mainly used for the treatment of anti-tumor and antiviral syndrome. Adverse reactions: TNFα may include fever, chills, headache, nausea, vomiting, loss of appetite, general fatigue, muscle soreness, etc.; high doses can cause shock, renal insufficiency, leukopenia and thrombocytopenia, and liver function changes. Eat red pigment. Used for cherry, fish cake, kelp fish roll, sausage, cake, fish floss, etc., the dosage is 5~100mg/kg. used as adsorption indicator and cell stain bacterial stain; Adsorption indicator for silver titration |
| production method | obtained by iodizing tetrachlorofluorescein and salting out with sodium chloride (refer to fluorescent pink). methods the engineering bacteria were inoculated into LB medium 1. the process of recombinant TNFα by bacterial fermentation, cultured to A600nm = 1 at 37 ℃, transferred to M9 medium, and induced at 42 ℃ for 4 hours. Centrifuge to collect bacteria. The bacteria were suspended with phosphate buffer solution (PB,10mmol/L) with pH 8, and the bacteria were broken by ultrasound, the supernatant was collected by centrifugation, and PB was dialyzed to obtain crude TNFα. Engineering bacteria [culture, fermentation] →[37 ℃, 42 ℃] fermentation bacteria [10mmol/L PB]→[pH8]TNFα crude product DEAE-Sepharose FF ion exchange column chromatography ion exchange column with PB balance, add TNFα crude product, wash the column with PB, add 75 mmol/L NaCl elution, collect each peak, measure TNFα activity. Crude TNFα [DEAE-Sepharose FF Ion Column] →[75 mmol/L NaCl]TNFα Active Component IQ-Sepharose FF Ion Exchange Column Chromatography Ion Exchange Column was balanced with pH 8 and 20 mmol/LTris-HCl buffer solution, then TNFα Active Component I was added, eluted with 80 mmol/L NaCl, each peak was collected, TNFα activity was measured, and TNFα active component II was obtained. TNFα Active Component I[Q-Sepharose FF Ion Column] →[80 mmol/L NaCl]TNFα Active Component IICM-Sepharose FF Ion Exchange Column Chromatography Ion Exchange Column is balanced with 10mmol/L PB with pH 6, TNFα Active Component II is added, and then the PB solution, PB solution, 200mmol/L NaCl and PB solution and 600 mmol/L NaCl are sequentially used for step-by-step elution, and each eluent is collected, the activity of TNFα was measured to obtain pure TNFα (at 4 ℃, through three atmospheric ion exchanges, electrophoresis showed that the elution peak of PB solution plus 600 mmol/L NaCl was a single band of TNFα, and pure TNFα was obtained). TNFα Active Component II[CM-Sepharose FF Ion Column] →[PB, 200 and 600mmol/L NaCl]TNFα Pure Product Method 2. Mutation Modification of Synthesized TNFα Mutation Modification Using Multi-site Mutation Primers and PCR Methods to Mutation Modification of Synthesized TNFα Gene Mutation Modification to Change its Second Arginine Codon CGT to Lysine Codon AAA to Probe Mutation Gene TNF-K2. TNFα gene [multi-site mutation primer, PCR method] → TNF-K2 transformation, recombination, insert TNF-K2 downstream of PL promoter of vector pSB-92, transform E.coliJM103, screen transformants by enzyme digestion, and obtain recombinant plasmid pSB-TNF-K2. TNF-K2[pSB-92]→ pSB-TNF-K2 culture, induction The pSB-TNF-K2 is cultured at 30 ℃ to logarithmic growth phase, and induced at 42 ℃ for 4 hours to obtain mutant [Lys2]TNFα, its molecular weight is 17kD, and its specific activity is 6.8 × 107U/mg protein. It is a soluble protein. pSB-TNF-K2[30 ℃, 42 ℃, 4h]→[Lys2]TNF α. |
| color index | 45440 |
| biological application | Apoptosis say; diagnosis of diseases related to amyloid accumulation; controlling plant diseases; identifying fungi; treating skin,mouth,digestive tract,urinary tract,reproductive tract,respiratory tract,circulatory system |
| EPA chemical information | information provided by: ofmpub.epa.gov (external link) |
Last Update：2024-04-10 22:29:15
Acid Red 94 - Production method
bacterial fermentation, the engineering bacteria were inoculated into LB medium, cultured at 37 ℃ to A600nm = 1, transferred to M9 medium, and induced at 42 ℃ for 4 hours. Centrifuge to collect bacteria. The bacteria were suspended with phosphate buffer solution (PB,10mmol/L) with pH 8, and the bacteria were broken by ultrasound, the supernatant was collected by centrifugation, and PB was dialyzed to obtain crude TNFα.
Engineering bacteria [culture, fermentation] & rarr;[37 ℃, 42 ℃] Fermentation bacteria [10mmol/L PB]& rarr;[pH8]TNFα crude
DEAE-Sepharose FF ion exchange column chromatography ion exchange column was balanced with PB, crude TNFα was added, the column was washed with PB, 75 mmol/L NaCl was added for elution, each peak was collected, and TNFα activity was measured.
Crude TNFα [DEAE-Sepharose FF ion column] & rarr;[75 mmol/L NaCl]TNFα active component I
Q-Sepharose FF ion exchange column chromatography ion exchange column was balanced with pH 8 and 20 mmol/LTris-HCl buffer, then TNFα active component I was added, eluted with 80 mmol/L NaCl, each peak was collected, TNFα activity was measured, and TNFα active component II was obtained.
TNFα active component I[Q-Sepharose FF ion column] & rarr;[80 mmol/L NaCl]TNFα active component II
CM-Sepharose FF ion exchange column chromatography ion exchange column is balanced with 10mmol/L PB with pH 6, TNFα active component II is added, and then the PB solution, PB solution, 200mmol/L NaCl and PB solution and 600 mmol/L NaCl are sequentially eluted step by step. Each eluent is collected and TNFα activity is measured to obtain TNFα pure product (at 4 ℃, through three atmospheric ion exchanges, electrophoresis shows that: the elution peak of PB solution plus 600 mmol/L NaCl is TNFα single band, and TNFα pure product can be obtained).
TNFα Active Component II[CM-Sepharose FF Ion Column] & rarr;[PB, 200 and 600mmol/L NaCl]TNFα Pure
Last Update：2024-04-10 22:29:15
Acid Red 94 - Nature
| solubility | H2O: soluble1mg/mL |
| color index | 45440 |
| maximum wavelength (& lambda;max) | 548 nm |
| sensitivity | Light Sensitive |
| Merck | 14,8262 |
| BRN | 3645857 |
| biological applications | Apoptosis assay; diagnosis of diseases related to amyloid accumulation; controlling plant diseases; identifying fungi; treating skin,mouth,digestive tract,urinary tract,reproductive tract,respiratory tract,circulatory system |
| EPA chemical information | Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 4,5,6,7-tetrachloro-3',6'-dihydroxy-2',4',5',7'-tetraiodo-, disodium salt (632-69-9) |
Last Update：2024-04-09 19:04:55
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View History
Raw Materials for Acid Red 94
Sodium chloride
Acid Red 92
Tetrachlorofluorescein
3,4,5,6-TETRACHLOROFLUORESCEIN
Tetrachlorophthalic anhydride
Resorcinol
Acid Red 92
Tetrachlorofluorescein
3,4,5,6-TETRACHLOROFLUORESCEIN
Tetrachlorophthalic anhydride
Resorcinol